Studies on the Mechanism of Oxidative Phosphorylation

Studies on the Mechanism of Oxidative Phosphorylation

Kinetic and nucleotide binding studies have shown that submitochondrial particles from bovine heart possess three exchangeable binding sites for ADP or GDP. In order of decreasing affinity at neutral pH, these sites will be referred to as sites I, II, and III, and their respective dissociation constants as KI, KIr, and KIII. In oxidative phosphorylation experiments in the presence of saturating...

Studies on the kinetic mechanism of oxidative phosphorylation.

The kinetics of the synthesis of ATP from ADP and Pi by beef heart submitochondrial particles were examined. When Pi was the variable substrate positive cooperativity was observed, whereas if ADP was varied, linear double reciprocal plots were obtained. The analog of Pi, thiophosphate, was a noncompetitive inhibitor of ATP synthesis with respect to ADP, while the analog of ADP, AMP (CH2)P, was ...

Studies on the Mechanism of Oxidative Phosphorylation. 8. Purification of the Reduced Cytochrome C Coupling Factor.

It seems likely that the mechanism of mitochondrial oxidative phosphorylation will be understood only after the components of the system have been separated and identified, and the mechanism of their action examined in detail. Experiments in this laboratory, therefore, have been directed toward the resolution of the oxidative phosphorylation system of beef heart mitochondria into its component ...

Further studies on oxidative photosynthetic phosphorylation.

" Studies on the mechanism of oxidative phosphorylation : Effects of specific FO modifiers on ligand - induced conformation changes of F 1

Aurovertin is a fluorescent antibiotic that binds to the catalytic 13 subunits of the mitochondrial FjATPase and inhibits ATP synthesis and hydrolysis. ATP, ADP, and membrane energization in submitochondrial particles (SMP) alter the fluorescence of Fl-bound aurovertin. These fluorescence changes are considered to be in response to the conformation changes of Fl-ATPase. This paper shows that th...